Platinum pfx dna polymerase manual

Platinum manual polymerase

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Complex templates include plant, human and other mammalian genomic DNA. Phusion DNA Polymerase is supplied with standard 5X Phusion HF platinum pfx dna polymerase manual Buffer, as well as 5X Phusion GC Buffer, which can be used for complex or GC-rich templates. 9-kb fragment with 66% amplification efficiency, which is significantly lower than the efficiency of Pfu alone (78%) or with dUTPase (83%).

Polymerase activity is restored after a PCR denaturation step at 94°C, providing an automatic “hot start” for. the prior express written permission of PLANET Technology. 1 µL of plasmid DNA (10 ng/µL, diluted in TE) was added to a 50 µL standard KOD Hot Start PCR reaction were determined on diluted. Product Description ∤ Platinum® Taq DNA Polymerase High Fidelity contains recombinant Taq DNA polymerase. Document Retrieval. Platinum pfx dna polymerase pdf Platinum pfx dna polymerase pdf Platinum pfx dna polymerase pdf DOWNLOAD!

Description PlatinumfiPfxDNA Polymerase is a proprietary enzyme preparation containing recombinant DNA polymerase from Thermococcussp. platinum pfx dna polymerase manual strain KOD (1,2). Resuspend each primer in Tris buffer pH 8. PCR - PLATINUM Pfx DNA Polymerase. Thermostable AccuPrime™ accessory proteins enhance specific primer-template hybridization during every cycle of platinum pfx dna polymerase manual PCR, increasing specificity, yield, and robustness over Platinum® Pfx alone. Human genomic DNA was obtained from BD Bio-sciences Clontech (La Jolla, CA).

323SIP VoIP Gateway: Model: VIP-281VIP-480VIP-1680VIP-2480 series. 10 µM forward primer 1 µL 0. Pfx50™ DNA Polymerase, 500rxn $ 824. PCR - PLATINUM Pfx DNA Polymerase Polymerase Description ()· Proofreading 3&39; à 5&39; exonuclease activity · Fast chain extension capability · One unit of Pfx pol incorporates 10 nmol of dNTP&39;s in 30 min at 74˚C. Platinum Pfx polymerase (Invitrogen catalogmM dNTPs in water (this means dATP=dCTP=dGTP=dTTP=10 mM; template DNA; 100 μM forward primer in water; 100 μM reverse primer in water; Procedure.

Platinum Pfx DNA polymerase amplified the 0. Platinum® Taq DNA Polymerase, 300rxn Polymerase chain reaction PCR was performed using 1 U of Platinum® Pfx DNA polymerase in a reaction containing 2 pg of the plasmid to be mutated, 1X Pfx buffer, 1 mM Mg2SO4, 0. Tgo DNA polymerase was purchased from Roche Molecular Biochemicals (Indianapolis, IN). 3 Add template DNA and primers Add your template DNA and primers to each tube for a final reaction volume of 50 μL. Thermostable AccuPrime accessory proteins enhance specific primer-template hybridization during every cycle of PCR, increasing specificity, yield, and robustness over Platinum Pfx alone.

6 Taq DNA polymerase 8. Platinum® Taq DNA Polymerase, 300rxn. The amplification was performed in a GeneAmp® PCR System 9700 with a initial denaturation at 94ºC (15. 2 kb amplicon Blunt Mutation % Probability‡ of a Correct Clone if You. 0 1X PfuTurbo® DNA Polymerase 0.

4 Vent R ® DNA polymerase 2. AFNS Stores > Bio Bars > Thermo Fisher / Invitrogen > Pfx50™ DNA Polymerase, 500rxn. Q5 = Q5 Hot Start High-Fidelity DNA Polymerase (NEB) PU = PfuUltra II Fusion HS DNA Polymerase (Agilent) PT = Platinum® Taq DNA Polymerase High Fidelity (Invitrogen/Life) PF = Phusion Hot Start Flex DNA Polymerase (NEB) AP = AccuPrime™ Pfx DNA Polymerase (Invitrogen/Life) 4,960 bp 370 bp 450 bp 410 bp 520 bp Amplification of a variety of. Vent® DNA Polymerase Blunt 23.

· 1 m l Pfx = 2. Shop a large selection of products and learn more about PLATINUM PFX DNA POLYMERASE. 3 mM dNTP mixture, 0. 8 pmol T7Prom and T7Term soluble primers, 1. Mix and then briefly centrifuge the components. TrackIt™ 1 Kb Plus DNA Ladder (Cat. High fidelity is provided by a proprietary enzyme preparation containing recombinant DNA polymerase from Thermococcusspecies KODwith proofreading (3&39;-5&39; exonuclease) activity. SYBR Green I, ROX, and DMSO1 were from Stratagene (La Jolla, CA).

To confirm that the DNA template had been successfully immobilized onto the beads, a second amplification reaction was performed in a final volume of 50 μl reaction mixture containing 1 mM MgSO4, 1X PCR buffer, 0. The protocols for setting up the PCR reaction and the cycling parameters for using Platinum® Taq DNA Polymerase High Fidelity and Platinum® Pfx DNA polymerase are provided on pages, respectively. Platinum Pfx DNA Polymerase is ideal for amplification of DNA fragments for high-fidelity PCR applications. Platinum pfx dna polymerase pdf Users Manual for PLANET 224-Port H. Phusion DNA Polymerase possesses 5´→ 3´ polymerase activity, 3´→ 5´ exonuclease activity and will generate blunt-ended products.

25 U Platinum pfx DNA polymerase (Invitrogen, NY, USA), distilled. Condition B: Pfx Polymerase Materials Required. 3 µM primer forward and 0. Hotstart DNA Polymerase – INSTRUCTION MANUAL – Catalog. Thermo Fisher platinum pfx dna polymerase AccuPrime Pfx DNA Polymerase is ideal for high fidelity amplification of DNA fragments for downstream applications such as cloning and mutagenesis High fidelity is provided by a proprietary enzyme preparation containing recombinant DNA polymerase from Thermococcus species KOD with proofreading 3 →5 exonuclease activity Platinum anti Pfx DNA. 3 mM dNTP mix, 0.

0 × 10. DNA polymerase 2. Platinum™SuperFi™DNA Polymerase is a proofreading DNA polymerase that combines fidelity with Platinum™hot-start technology, and is ideally suited for cloning, mutagenesis, and other applications. 3 µM primer reverse. • Platinum® Pfx DNA Polymerase: a high-fidelity DNA polymerase with automatic hot-start for oligonucleotide assembly and amplification • DNA Quantitation Module: an ultra sensitive fluorescent nucleic acid stain for measuring the concentration of double-stranded DNA (dsDNA). DNA polymerase and 18-fold lower than Taq DNA polymerase, making it the highest fidelity enzyme available (See Table I).

PLATINUM® Pfx DNA polymerase 3. Product description The Invitrogen™Platinum Pfx DNA Polymerase is a proprietary enzyme preparation containing recombinant DNA polymerase from Thermococcus species KOD. * Phusion DNA Polymerase was developed by Finnzymes Oy, now a part of Thermo Fisher Scientific.

Platinum® anti- Pfx DNA polymerase antibodies inhibit polymerase activity, providing hot-start capabilities for improved PCR specificity (see figure). AccuPrime Pfx DNA Polymerase is ideal for high-fidelity amplification of DNA fragments for downstream applications such as cloning and mutagenesis. 5 Values provided by individual manufacturers. Platinum Pfx DNA Polymerase is ideal for amplification of DNA fragments for high-fidelity PCR.

6-kb fragments) DNA polymerase due to failure to amplify. Platinum SuperFi DNA Polymerase is engineered with a DNA-binding domain resulting in high processivity and increased resistance to PCR inhibitors. 0 or distilled water to 100 µM.

Other polymerases that generate blunt ends or 3′ A- overhangs may be suitable. Although Pfx DNA polymerase is blended with neutralizing antibody, the results shown here for 10 d ifferent amplification systems, of varying lengths and manual complexities, indicate that PfuTurbo DNA polymerase provides comparable, if not superior (Figure 3), specificity compared to Platinum Pfx DNA polymerase. Platinum® Pfx DNA Polymerase is provided in inactive form, due to specific binding of the Platinum® antibody. 6 PLATINUM ® Pfx DNA polymerase 3. 30 Taq DNA Polymerase 3&39;-A 67. Product description The Invitrogen™Platinum Pfx DNA Polymerase is a proprietary enzyme preparation containing recombinant DNA polymerase from Thermococcus species KOD. • Due to the nature of the Phusion™ High–Fidelity DNA Polymerase, the optimal reaction conditions may differ from PCR protocols for standard DNA polymerases.

5% of full-length PCR products, making PfuUltra high-fidelity DNA polymerase the ideal enzyme for PCR cloning. Platinum™ Taq DNA Polymerase 0. The Platinum™Pfx DNA Polymerase possesses proofreading 3¢ to 5¢ exonuclease activity and is a highly processive enzyme with fast chain extension capability. Platinumanti- Pfx DNA polymerase antibodies inhibit polymerase activity, providing hot-start capabilities for improved PCR platinum pfx dna polymerase manual specificity. Polymerase Description · Proofreading 3&39; à 5&39; exonuclease activity · Fast chain extension capability · One unit of Pfx pol incorporates 10 nmol of dNTP&39;s in 30 min at 74˚C. 42 17X Platinum® Pfx DNA Polymerase 0. 2 µL 2 U/rxn *For targets >5 kb or with >65% GC sequences. 3 Template not specified.

or 3&39;-A Frequency Sequence Sequence Sequence. This feature also enables fast-cycling protocols and amplification of long targets. Taq DNA Polymerase 4.

0 KOD DNA polymerase 3. Amplification of a 500-bp fragment using PfuUltra high-fidelity DNA polymerase results in errors in less than 0. coli genomic DNA. 4 Simple templates include plasmid, viral and E. Super Pfx DNA Polymerase is a fast, high-efficiency, high-fidelity DNA polymerase with 5&39;-3&39; DNA polymerase activity and 3&39;-5&39; exonuclease activity. polymerases were from Stratagene. 5-mL nuclease-free microcentrifuge tubes Timing Varies depending on amplicon length Selection Guide PCR Enzymes and Master Mixes Go online to view related products. Platinum® Pfx DNA Polymerase amplifies genomic templates up to 12 kb and plasmid templates up to 20 kb, and is ideal for demanding PCR applications such as site-directed mutagenesis and expression cloning.

9-kb fragments) or Tgo (>0. Platinum Taq DNA Hi-Fi ú AccuPrime Taq æäÚ(þ) AccuPrime™ Pfx DNA Polymerase (ÙV÷VøVû(ü æäëVì(í, öîóVÚVÛ æäÿ Vú VÛVù; formulated with Pfx DNA Polymerase, Platinum "hot-sta r" Pl inu m- fx b od e, A c y p ). Efficiencies could not be determined for longer targets using Platinum Pfx (>0.

This polymerase is modified from other high-fidelity enzymes, has strong amplification ability, rapid amplification speed (4-6 kb/min), and high fidelity. PfuUltra Hotstart DNA Polymerase Instruction Manual Catalog 600390, 600392, and 600394. Component 50-µL rxn Final conc. High fidelity is provided by a proprietary enzyme preparation containing recombinant DNA polymerase from Thermococcus species KOD with proofreading (3&39;-5&39; exonuclease) activity. Platinum® anti-Pfx DNA polymerase antibodies inhibit polymerase activity, providing hot-start capabilities for improved PCR specificity. ∤ 0. PlatinumfiPfxDNA Polymerase possesses proofreading 3′ to 5′ exonuclease activity and provides higher fidelity than PfuDNA polymerase (3). • The Phusion™ High–Fidelity DNA Polymerase should be pipetted carefully and gently as the high glycerol content (50%) in the storage buffer may otherwise lead to pipetting errors.

0 a Fidelity is measured using a published PCR forward mutation assay that is based on the lacI target gene. 70 Platinum® Pfx DNA Polymerase Blunt 29. Platinum Taq and Platinum Pfx were purchased from Invitrogen. High fidelity is provided by a proprietary enzyme preparation containing recombinant DNA polymerase.

Platinum pfx dna polymerase manual

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